Uji Efektivitas Daya Hambat Air Perasan Jeruk Nipis (Citrus aurantiifolia, Swingle) Terhadap Pertumbuhan Bakteri Stahpylococcus Aureus ATCC 25923 Secara In Vitro

Purwani, Ni Luh Ayumas Oktavia (2018) Uji Efektivitas Daya Hambat Air Perasan Jeruk Nipis (Citrus aurantiifolia, Swingle) Terhadap Pertumbuhan Bakteri Stahpylococcus Aureus ATCC 25923 Secara In Vitro. S1 thesis, Universitas Kristen Indonesia.

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Abstract

Staphyloccocus aureus adalah bakteri flora normal pada manusia yang paling sering ditemukan pada bagian hidung. Infeksi pada sistem pernafasan seperti pneumonia, ispa, dan influenza. Penggunaan antibiotik yang cukup tinggi dan tidak dapat dikendalikan merupakan faktor yang dapat menyebabkan resistensi terhadap pengobatan. Maka dari itu pemberian obat herbal adalah alternatif. Salah satu buah jeruk nipis yang mengatakan memiliki efek antibakteri sebagai reaksi antibiotik. Tujuan penelitian ini adalah Mengetahui Efektivitas Udara Perasan Jeruk Nipis terhadap pertumbuhan bakteri Staphylococcus aureus ATCC 25923 . Desain penelitian ini adalah eksperimental dengan metode Postest Only Control Group Design. Sampel yang digunakan adalah bakteri Staphylococcus aureus ATCC 25923 yang disebar pada agar Mueller Hinton yang kemudian diberi perlakuan dengan udara perasan jeruk nipis dengan beberapa konsentrasi yaitu 20%, 40%, 60%, 80% dan 100%, dan menggunakan kontrol negatif adalah akuades steril dan kontrol positif adalah disk ciproxim. Hasil penelitian didapatkan rata-rata luas zona hambat perasan air jeruk nipis terhadap Staphylococcus aureus ATCC 25923 yaitu pada konsentrasi 20% didapatkan diameter zona hambat 6,18 mm, pada konsentrasi 40% didapatkan diameter zona hambat 6,34 mm, pada konsentrasi 60% didapatkan diameter zona hambat 6,34 mm, pada konsentrasi 80% didapatkan diameter zona hambat 7,11 mm, dan pada konsentarsi 100% didapatkan diameter zona hambat 11,26 mm. Kata kunci: Air perasan jeruk nipis, Staphylococcus aureus, antibakteri Staphyloccocus aureus is a normal bacterial flora in humans that is most often found in the nose. Infection of the respiratory system such as pneumonia, ispa, and influenza. The use of antibiotics that are quite high and cannot be controlled is therefore the provision of herbal medicines is an alternative. One of the lime fruits which says has an antibacterial effect as an antibiotic reaction. The purpose of this study was to find out the effectiveness of air lime juice on the growth of Staphylococcus aureus bacteria ATCC 25923 . The design of this study was experimental with the Postest Only Control Group Design method. The sample used was Staphylococcus aureus bacteria ATCC 25923 which was spread on Mueller Hinton agar which was then treated with lime juice with several concentrations of 20%, 40%, 60%, 80% and 100%, and using negative controls was sterile aquades and controls positive is the ciproxim disk. The results showed that the average zone of inhibition of lime juice on Staphylococcus aureus ATCC 25923 was at a concentration of 20% obtained by inhibition zone diameter of 6.18 mm, at a concentration of 40% obtained by inhibition zone diameter of 6.34 mm, at a concentration of 60% obtained zone diameter inhibition of 6.34 mm, at a concentration of 80% the diameter of the inhibition zone was 7.11 mm, and at 100% concentration the diameter of the inhibition zone was 11.26 mm. Key words: Lime juice, Staphylococcus aureus, antibacterial

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