THE RELATIONSHIP BETWEEN THE RATE OF DECREASE IN BODY TEMPERATURE AND THE APPEARANCE OF CORPSE STIFFNESS IN WISTAR RATS THAT DIED DUE TO BLOOD LOSS

Wijoyo, Suryo (2022) THE RELATIONSHIP BETWEEN THE RATE OF DECREASE IN BODY TEMPERATURE AND THE APPEARANCE OF CORPSE STIFFNESS IN WISTAR RATS THAT DIED DUE TO BLOOD LOSS. International Journal of Modern Pharmaceutical Research, 6 (1). pp. 36-45. ISSN 2319 5878

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Abstract

Every death will be accompanied by signs of death such as rigour Mortis, Algor mortis, livor mortis, and decomposition. In death with massive blood loss, Algor Mortis dan rigour Mortis should appear faster than death without blood loss. It will affect the death estimated time by a forensic doctor. This study is based on the discipline of Physiology and Forensic Sciences that are trying to evaluate Mortis and Algor Mortis in mice as an initial study to look at the relationship between blood loss and signs of definite death. The number of experimental animals used is calculated using the formula experimental Freeder size: (n-1) (t-1) ≥ 15, in which the required sample size is 16 samples divided into two groups of experimental animals. In both groups, we perform anaesthesia using ketamine at a 50-75 mg/kg dose and xylazine at 10 mg/kg administered intraperitoneally (I.P.). The control group will be subject to cervical dislocation, while the other group will have blood drawn as much as ± 30% of the total blood to trigger hypovolemic shock using cardiac puncture techniques. When cervical dislocation is done in both groups, the time required to decrease body temperature and the appearance of rigour Mortis will be observed in both experimental groups. From the research that has been done, it can be concluded that blood loss may be one of the factors that affect the speed of the decline in body temperature of mice to the ambient temperature and the speed of the formation of rigour Mortis. It was proven using Kendall’s Tau nonparametric correlation with the results -,770 for body temperature and Pearson correlation with the results -,891 for Algor Mortis. The group that had blood drawn more quickly reached ambient temperature by 19,8 % compared to the group who did not have blood drawn, and groups who had blood drawn reached rigour Mortis maximum score of 32% are faster than the group that did not have blood drawn.

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