The Effect of Sperm DNA Fragmentation on Fertilization and Embryogenesis Rates

Sirait, Batara I. (2020) The Effect of Sperm DNA Fragmentation on Fertilization and Embryogenesis Rates. Solid State Technology, 63 (1). pp. 1459-1471. ISSN 0038 111X

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Abstract

This research is the effects of sperm DNA fragmentation on fertilization and embryogenesis rates. The purpose of doing this research is to find out the effects of sperm DNA fragmentation on fertilization and embryogenesis rates. It is a quantitative study with cross-sectional design and carried out by taking male semen samples from infertile couples who participated in the IVF-ICSI program, examined WHO sperm analysis, and assessed the sperm DNA fragmentation index. For the analysis of the mean difference of 2 categories using independent sample t-tests while those of more than 2 categories use Anova. Analysis of categorical variable types uses the chi-square test. The result of this research shows that In the 10-month study period, the number of study subjects who met the inclusion criteria was 29 infertile couples with 29 ejaculate sperm samples and 200 oocytes. From the analysis of sperm DNA fragmentation index with fertilization rates, it appears that good fertilization (Z1 and Z2) is more common in groups with sperm DNA fragmentation index 30%, and statistically significantly different with p <0.05, and from analysis of sperm DNA fragmentation index with embryo scoring seen better embryo scoring (grades A and B) in the sperm DNA fragmentation index group 30%, which was not statistically significant. It is concluded that the sperm DNA fragmentation rates can show a negative relationship with fertilization rates but can not show a statistically significant relationship with embryogenesis rates. A statistically significant relationship was seen between the World Health Organization (WHO) sperm analysis parameters and DNA fragmentation index examined using the SCD method.

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