Efektivitas Antibakteri Ekstrak antara Daun Jeruk Nipis (Citrus Aurantifolia Swingle) dan Daun Jeruk Purut (Citrus Hystrix Dc) terhadap Escherichia Coli

Kaban, Astari Kinanti Karina br (2018) Efektivitas Antibakteri Ekstrak antara Daun Jeruk Nipis (Citrus Aurantifolia Swingle) dan Daun Jeruk Purut (Citrus Hystrix Dc) terhadap Escherichia Coli. S1 thesis, Universitas Kristen Indonesia.

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Abstract

Penggunaan obat tradisional sebagai alternatif banyak diterapkan di Indonesia. Salah satunya adalah penggunaan daun jeruk nipis dan daun jeruk purut sebagai obat tradisional. Peneliti tertarik untuk mengetahui efektivitas antibakteri ekstrak antara daun jeruk nipis dan daun jeruk purut terhadap Escherichia coli ATCC 25922. Penelitian ini merupakan penelitian eksperimental desktriptif. Daun jeruk nipis dan daun jeruk purut yang diekstraksi menggunakan metode maserasi dan diuji sensitivitas menggunakan metode Kirby Bauer terhadap pertumbuhan Escherichia coli ATCC 25922 dengan konsentrasi 10%, 30%, 50%, 70%, dan 100%. Hasil pengujian menunjukkan bahwa ekstrak daun jeruk nipis dan daun jeruk purut dapat menghambat pertumbuhan bakteri Escherichia coli. Penghambatan tertinggi adalah daun jeruk nipis pada konsentrasi 100% (13,25 mm). Tidak ada perbedaan yang mencolok dari zona hambatan daun jeruk nipis dan daun jeruk purut terhadap Escherichia coli. Kata kunci : Daun jeruk purut (Citrus hystrix DC), daun jeruk nipis (Citrus aurantifolia Swingle), Escherichia coli, metode Kirby Bauer. / The use of traditional medicine as alternatives has been used many times in Indonesia. The usage of lime and kaffir lime are several examples of traditional medicine. The researcher is interested in knowing the antibacterial effects between lime leaves extract and kaffir lime leaves extract towards Escherichia coli ATCC 25922. This research is an experimental descriptive research. Lime leaves and kaffir lime leaves was extracted by maseration and its sensitivity towards the growth of Escherichia coli ATCC 25922 tested via Kirby Bauer method with 10%, 30%, 50%, 70%, and 100% extracts. Test results shows that lime leaves extract and kaffir lime leaves extract can inhibit the growth of Escherichia coli. The greatest suspension was lime leaves extract at 100% concentration (13,25 mm). There was no significant difference between the inhibitory zone of lime leaves extract and kaffir lime leaves extract towards Escherichia Coli Keywords: kaffir lime leaves (Citrus hystrix DC), lime leaves (Citrus aurantifolia), Escherichia coli, Kirby Bauer method.

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