Aktivitas Antioksidan dan Antibakteri Ekstrak Buah Parijoto (Medinilla speciosa Blume)

Kristanti, Agata (2023) Aktivitas Antioksidan dan Antibakteri Ekstrak Buah Parijoto (Medinilla speciosa Blume). S1 thesis, Universitas Kristen Indonesia.

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Abstract

Penyakit yang timbul akibat radikal bebas dan infeksi semakin meningkat. Berbagai upaya dilakukan oleh masyarakat untuk memelihara kesehatannya, salah satunya adalah memanfaatkan tanaman obat. Parijoto (Medinilla speciosa Blume) menjadi salah satu tanaman obat yang dimanfaatkan oleh masyarakat Indonesia, khususnya warga desa Colo-Kudus, Jawa Tengah. Penelitian terdahulu mengungkapkan bahwa buah parijoto mengandung senyawa aktif flavonoid, saponin, tanin, alkaloid, dan glikosida sebagai antioksidan dan bersifat antibakteri. Tujuan penelitian ini untuk menguji dan mengetahui pengaruh ekstrak metanol buah parijoto sebagai antioksidan dan antibakteri terhadap pertumbuhan kuman Gram negatif (S. dysenteriae dan K. pneumoniae) dan kuman Gram positif (S. pyogenes dan MRSA). Penelitian bersifat eksperimental dengan metode DPPH untuk uji antioksidan dan metode Kirby-Bauer untuk uji antibakteri. Hasil menunjukkan ekstrak metanol buah parijoto mempunyai aktivitas antioksidan dengan nilai IC50 1,856 ppm dan pada konsentrasi 12,5 mg/ml, 25 mg/ml, 50 mg/ml, dan 100 mg/ml mempunyai aktivitas antibakteri yang mampu menghambat pertumbuhan kuman S. pyogenes dengan rerata diameter zona inhibisi 8,68 mm; 9,6 mm; 10,42 mm; 11,72 mm dan MRSA dengan rerata diameter zona inhibisi 7,43 mm; 8,43 mm; 9,35 mm; 12,07 mm, namun tidak mampu menghambat pertumbuhan kuman S. dysenteriae dan K. pneumoniae. Ekstrak metanol buah parijoto mempunyai aktivitas antioksidan sangat kuat dan mempunyai aktivitas antibakteri yang tidak efektif terhadap kuman Gram negatif (S. dysenteriae dan K. pneumoniae) serta efektivitas lemah terhadap kuman Gram positif (S. pyogenes dan MRSA). Kata kunci: antioksidan, antibakteri, Medinilla speciosa Blume, S. dysenteriae, K. pneumoniae, S. pyogenes, MRSA. / Diseases occurred by free radical and infection are increasing. Various ways have been done by many people to preserve their health, one of them is by using medicinal plants. Parijoto (Medinilla speciosa Blume) is one of medicinal plants which used by Indonesian society, especially rural people in Colo-Kudus, Central Java. Previous research revealed that parijoto fruit contains active compounds such as flavonoids, saponins, tannin, alkaloids, and glycosides as antioxidant and antibacterial. The purposes of this research are to examine and determine the effect of methanol extract of parijoto fruit as antioxidant and antibacterial on the growth of negative Gram bacteria (S. dysenteriae and K. pneumoniae) and positive Gram bacteria (S. pyogenes and MRSA). This is an experimental research with DPPH method for antioxidant test and Kirby-Bauer method for antibacterial test. The results show that methanol extract of parijoto fruit has antioxidant activity with IC50 value 1,856 ppm and at concentration 12,5 mg/ml, 25 mg/ml, 50 mg/ml, and 100 mg/ml has antibacterial activity which afford to inhibit S. pyogenes bacteria with average diameter of inhibition zone 8,68 mm; 9,6 mm; 10,42 mm; 11,72 mm and MRSA with average diameter of inhibition zone 7,43 mm; 8,43 mm; 9,35 mm; 12,07 mm, however the extract is incapable to inhibit the growth of S. dysenteriae and K. pneumoniae bacteria. Methanol extract of parijoto fruit has overpowering antioxidant activity and has ineffective antibacterial effect on negative Gram bacteria (S. dysenteriae and K. pneumoniae) and weak effectiveness on positive Gram bacteria (S. pyogenes and MRSA). Keywords: antioxidant, antibacterial, Medinilla speciosa Blume, S. dysenteriae, K. pneumoniae, S. pyogenes, MRSA

Item Type: Thesis (S1)
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorArodes, Evy SuryaniNIDN0308048803evy.arodes@uki.ac.id
Additional Information: Nomor Panggil : T.A 628.16 Aga a 2023
Subjects: MEDICINE
MEDICINE > Botanic, Thomsonian, and eclectic medicine
Divisions: FAKULTAS KEDOKTERAN > Pendidikan Dokter
Depositing User: Users 2942 not found.
Date Deposited: 22 May 2023 04:55
Last Modified: 11 Jul 2023 07:45
URI: http://repository.uki.ac.id/id/eprint/11289

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