Toxicity Test of Strong Drug Using the BSLT (Brine Shrimp Lethality Test) Method

Pohan, Dame Joyce and Marantuan, Randolph Serep and Djojosaputro, Mulyadi (2023) Toxicity Test of Strong Drug Using the BSLT (Brine Shrimp Lethality Test) Method. International Journal of Health Sciences and Research, 13 (2). pp. 203-209. ISSN 2249-9571

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Abstract

One of the methods to test cytotoxic materials is to test the toxicity of Artemia salina leach shrimp larvae (Brine Shrimp Lethality Test). This method is often used for pre-screening the active compounds contained in plant extracts because it is cheap, fast, easy (no need for aseptic conditions), and trustworthy. This study aims to determine the acute toxic effects of the powerful drugs used by the public. This research was experimental research using Post Test Only Control Group Design. It used 120 larvae as test animals which were divided into four groups. Each group contains ten larvae. Each group is done by the replication of research two times. As the test component, the cause of bitter melon is given through the media, which contains larvae as the animal test. The final extract concentration in the media, which contains larvae consecutively as the group of 1, is 4000 ppm, 2000 ppm, and 0 ppm for negative control. The result is against larvae that died 24 hours after the component test was given. Through the data, the LC50 value of strong herb was analyzed by probit analysis using Excel for windows. This study shows the load concentration extract in a medium can kill the larvae in a row with a concentration of 4000 ppm and 2000 ppm. The results of probit analysis showed that there is no toxicity to Artemia salina leach larvae. Then it is concluded that there is no potential for acute toxicity according to Artemia salina leach when it was tested using BLST. Key words: brine shrimp lethality test, herbal, strong medicine, acute toxicity, Artemia salina leach.

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