Uji Efektivitas Ekstrak Bawang Putih (Alliumsativum L.) Dalam Berbagai Konsentrasi Terhadappertumbuhan Kuman Staphylococcus Aureus

Gustiarini, Dini (2018) Uji Efektivitas Ekstrak Bawang Putih (Alliumsativum L.) Dalam Berbagai Konsentrasi Terhadappertumbuhan Kuman Staphylococcus Aureus. S1 thesis, Universitas Kristen Indonesia.

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Abstract

Bawang putih (Allium sativum L.) dipercaya sebagai tanaman obat yang dapat mengobati berbagai penyakit, salah satunya sebagai antibakteri. Bawang putih mengandung golongan senyawa allisin, flavonoid, minyak atsiri, dan ajoene yang memiliki efek antibakteri terhadap Staphylococcus aureus. Staphylococcus aureus merupakan salah satu kuman yang berperan terhadap munculnya penyakit akne vulgaris. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak bawang putih (Allium sativum L.) terhadap pertumbuhan Staphylococcus aureus. Pada penelitian ini, 3 kg bawang putih diekstraksi dengan menggunakan etanol pro analisis sampai didapatkan ekstrak seperti minyak kental. Ekstrak bawang putih dengan konsentrasi 10%, 20%, 40%, 80%, dan 100% serta kontrol positif dengan antibiotik ciproxin dan kontrol negatif dengan akuades steril diuji aktivitas antibakterinya terhadap pertumbuhan Staphylococcus aureus dengan metode disc diffusion pada media Mueller-Hinton Agar. Didapatkan hasil bahwa ekstrak bawang putih dapat menghambat pertumbuhan Staphylococcus aureus, hasilnya menunjukkan bahwa semakin besar konsentrasi ekstrak bawang putih semakin kuat daya hambat terhadap pertumbuhan kuman. Berdasarkan analisis data dengan metode Kruskall – Wallis menunjukkan perbedaan yang bermakna antar setiap konsentrasi ekstrak bawang putih dengan kontrol positif berupa ciproxin. Kata kunci: Bawang putih, Staphylococcus aureus, Antibakteri, disc diffusion Garlic (Allium sativum L.) has been used as an medicinal plant to treat various diseases, one of them is antibacterial. Garlic is containing several compounds, such as allicins, flavonoids, essential oils, and ajoene which have an antibacterial effect on Staphylococcus aureus. Staphylococcus aureus is one of the germs that play a role in causing acne vulgaris. This study was conducted to determine the effect of garlic extract (Allium sativum L.) to Staphylococcus aureus growth. In this study, 3 kg garlic was extracted using ethanol pro analysis, and producing viscously oil extract. Garlic extract with various concentrations 10%, 20%, 40%, 80%, and 100% were tested on Mueller-Hinton Agar by using disc diffusion method to determine antibacterial activity to Staphylococcus aureus growth. Ciproxin and sterile aquades were used as positive and negative controls, respectively. This study showed garlic extract has inhibitory effect to Staphylococcus aureus growth. The greater concentration of the extract produces stronger inhibition on the growth of Staphylococcus aureus. Results were compared using Kruskall – Wallis method and showed a significant difference between each concentration with positive controls. Key words: Garlic, Staphylococcus aureus, antibacteria, disc diffusion

Item Type: Thesis (S1)
Subjects: SCIENCE > Botany
SCIENCE > Microbiology > Bacteria
Divisions: FAKULTAS KEDOKTERAN > Pendidikan Dokter
Depositing User: Ms Sari Mentari Simanjuntak
Date Deposited: 27 Jan 2020 03:04
Last Modified: 27 Jan 2020 03:04
URI: http://repository.uki.ac.id/id/eprint/1173

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