Efek Ekstrak Daun Putri Malu (Mimosa Pudica Linn) Sebagai Penghambat Pertumbuhan Vibrio Cholerae

Sitanaya, Juan Rizky (2021) Efek Ekstrak Daun Putri Malu (Mimosa Pudica Linn) Sebagai Penghambat Pertumbuhan Vibrio Cholerae. S1 thesis, Universitas Kristen Indonesia.

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Abstract

ndonesia terkenal dengan keanekaragaman sumber nabati yang bermanfaat bagi kesehatan, salah satunya adalah tumbuhan Putri Malu (Mimosa pudica Linn). Daun Putri Malu mengandung senyawa seperti alkaloid, flavonoid, saponin, dan tanin yang bersifat antibakteri. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun Putri Malu sebagai penghambat pertumbuhan bakteri Vibrio cholerae. Penelitian ini bersifat eksperimental secara in vivo dengan metode Kirby-Baurer disk diffusion. Konsentrasi ekstrak daun Putri Malu yang digunakan adalah 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml, 6,25 mg/ml, 3,125 mg/ml, dan 1,5625 mg/ml. Sebagai kontrol positif digunakan antibiotik tetrasiklin 30 mcg dan aquadest sebagai kontrol negatif. Diameter ratarata zona hambat bakteri Vibrio cholerae adalah 21,00 mm dan 0 mm masingmasing untuk kontrol positif dan kontrol negatif. Rata-rata diameter zona hambat bakteri terhadap ekstrak daun Putri Malu secara berurutan adalah 2,36 mm, 1,26 mm, 0,96 mm, 1,30 mm, 0,80 mm, 0,60 mm, dan 0,36 mm. Berdasarkan data dapat disimpulkan ekstrak daun putri malu tidak efektif dalam menghambat pertumbuhan bakteri Vibrio cholerae. Kata kunci: daun Putri Malu, Vibrio cholerae, aktivitas antibakteri. / Indonesia is famous for its diversity of vegetable resources that are beneficial for health, one of which is the Putri Malu plant (Mimosa pudica Linn). Mimosa pudica Linn leaves contain compounds such as alkaloids, flavonoids, saponins, and tannins that are antibacterial. This study aims to find out the effects of ethanol extract of Mimosa pudica Linn leaves as a growth inhibitor of Vibrio cholerae bacteria. This research is experimental in vivo with Kirby-Baurer disk diffusion method. The concentrations of Mimosa pudica Linn leaf extract used are 100 mg/ml, 50 mg/ml, 25 mg/ml, 12,5 mg/ml, 6,25 mg/ml, 3,125 mg/ml, and 1,5625 mg/ml. As a positive control used tetracycline antibiotics 30 mcg and aquadest as a negative control. The average diameter of Vibrio cholerae bacterial bland zone is 21.00 mm and 0 mm for positive control and negative control respectively. The average diameter of the bacterial bland zone against Mimosa pudica Linn leaf extract in a sequence is 2.36 mm, 1.26 mm, 0.96 mm, 1.30 mm, 0.80 mm, 0.60 mm, and 0.36 mm. Based on the data can be concluded Mimosa pudica Linn leaf extract is ineffective in inhibiting the growth of Vibrio cholerae bacteria. Keyword: Mimosa pudica Linn leaves, Vibrio cholerae, antibacterial activity.

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