Insidensi dan Identifikasi Infeksi Metichilin-Resistant Staphylococcus Aureus (MRSA) pada Mahasiswa Fakultas Kedokteran Universitas Kristen Indonesia

Paipinan, Sergio (2019) Insidensi dan Identifikasi Infeksi Metichilin-Resistant Staphylococcus Aureus (MRSA) pada Mahasiswa Fakultas Kedokteran Universitas Kristen Indonesia. S1 thesis, Universitas Kristen Indonesia.

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Abstract

Staphylococcus aureus merupakan flora normal yang dapat berubah menjadi patogen ketika seseorang mengalami kondisi imunokompromais. Salah satu permasalahan yang ditimbulkan oleh Staphylococcus aureus yaitu resistensi pengobatan terhadap antibiotik golongan β-laktam termasuk penicillin dan turunannya. Kegagalan pengobatan ini memunculkan galur baru spesies Staphylococcus aureus yang dikenal sebagai Metichilin-Resistant Staphylococcus aureus (MRSA). Penelitian ini bertujuan untuk mengidentifikasi dan mendeteksi kemungkinan karier MRSA pada mahasiswa angkatan 2016 Fakultas Kedokteran Universitas Kristen Indonesia. Metode yang digunakan yaitu eksperimental observasional dengan 30 sampel isolat swab nasal. Hasil penelitian didapatkan sebanyak 3 sampel (10%) dinyatakan positif karier MRSA setelah dilakukan uji kepekaan dengan media Müller-Hinton Agar (MHA) menggunakan metode disk diffusion test Kirby-Baüer, antibiotik yang digunakan yaitu Cefoxitin 30 μg (Oxoid Limited, Hampshire, UK), zona hambat yang terbentuk diukur menurut standar pedoman Clinical and Laboratory Standart Institute (CLSI) dan European Committee on Antimicrobial Susceptibility Testing (EUCAST). Kata kunci: Karier, MRSA, Mahasiswa Kedokteran, Indonesia, Staphylococcus aureus. / Staphylococcus aureus is a normal flora that can turn into a pathogen when a person experiences an immunocompromised condition. One of the problems caused by Staphylococcus aureus is treatment resistance to β-lactam antibiotics including penicillin and its derivatives. This treatment failure led to a new strain of Staphylococcus aureus species known as Metichilin-Resistant Staphylococcus aureus (MRSA). This study aims to identify and detect the possibility of MRSA careers in the 2016 batch of Medical Faculty students at the Indonesian Christian University. The method used is observational experimental with 30 samples of nasal swab isolates. The results obtained as many as 3 samples (10%) tested positive for MRSA careers after a sensitivity test with Müller-Hinton Agar (MHA) using the Kirby-Baüer disk diffusion test method, the antibiotic used was Cefoxitin 30 μg (Oxoid Limited, Hampshire, UK ), the inhibition zones formed are measured according to the Clinical and Laboratory Standards Institute (CLSI) guidelines and the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Keywords: Careers, MRSA, Medical Student, Indonesia, Staphylococcus aureus.

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