Uji Aktivitas Antibakteri Ekstrak Etanol Lidah Buaya (Aloe Vera) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus

Manurung, Ruth Yosephine Grace Uly Maharani (2020) Uji Aktivitas Antibakteri Ekstrak Etanol Lidah Buaya (Aloe Vera) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus. S1 thesis, Universitas Kristen Indonesia.

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Abstract

Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak lidah buaya dalam menghambat pertumbuhan bakteri S. aureus. Penelitian eksperimental ini menggunakan pendekatan Postest Only Control Group Design. Daun lidah buaya diekstraksi di Laboratorium PAU (Pusat Antar Universitas), Institut Pertanian Bogor dan diuji efektifitasnya dalam menghambat pertumbuhan bakteri S. aureus. Analisis data menggunakan cara One-way ANOVA dilanjutkan dengan post-hoc LSD. Hasil menunjukkan bahwa ekstrak Aloe vera konsentrasi 20% dan 40% masih belum menunjukkan adanya zona hambat. Daya hambat ekstrak lidah buaya konsentrasi 60%, 80% dan 100% masing-masing adalah 6.5±0.53 mm, 6.8±0.58 mm, dan 7.3±0.84 mm, and ketiga konsentrasi ini tidak berbeda secara statistik (p>0,05). Jika dibandingkan dengan kontrol (+) kloramfenikol, efektivitas ekstrak lidah buaya lebih rendah (p<0,05). Dapat disimpulkan bahwa pemberian ekstrak lidah buaya dapat menghambat pertumbuhan bakteri S. aureus, dengan onsentrasi minimum 60%. Kata kunci: Ekstrak lidah buaya, S. aureus, antibakteri, zona hambat. / The purpose of this study is to determine the effectiveness of aloe vera extract in inhibiting the growth of S. aureus bacteria. This experimental study used Postest Only Control Group Design. Aloe vera leaves wre extracted at the Laboratory of Inter-University Center, Bogor Agricultural University and its effectivity againts Staphylococcus aureus growth was examined in vitro. Data were then analyzed using One-way ANOVA followed by post-hoc LSD test. The results showed that Aloe vera extract of 20% and 40% did not show any inhibitory zones. Additionally, the inhibitory zones of 60%, 80% and 100% Aloe vera extract were observed at 6.5 ± 0.53 mm, 6.8 ± 0.58 mm, and 7.3 ± 0.84 mm, respectively, and these three concentrations did not differ statistically (p> 0.05). When compared with the positive control (chloramphenicol), the effectiveness of Aloe vera extract was lower (p <0.05). Therefore, it is suggesting that the administration of Aloe vera extract can inhibit the growth of Staphylococcus aureus bacteria, with a minimum concentration of 60%. Keywords: Aloe vera extract, Staphylococcus aureus, antibacterial, inhibitory zone.

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