Aktivitas Antioksidan Ekstrak Ranting Lagundi (Vitex Negundo L)

Sihombing, Pretty Oktaviani (2019) Aktivitas Antioksidan Ekstrak Ranting Lagundi (Vitex Negundo L). S1 thesis, Universitas Kristen Indonesia.

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Abstract

Uji aktivitas antioksidan dari ekstrak ranting Vitex negundo L dengan metode 1,1- difenil-2-pikrilhidrazil dilakukan dengan tujuan untuk mengetahui kekuatan antioksidan dari ekstrak ranting lagundi. Ranting yang digunakan dalam penelitian ini adalah ranting yang segar. Variasi konsentrasi ekstrak ranting lagundi adalah 10 ppm, 50 ppm, 100 ppm, 150 ppm dan 200 ppm. Larutan yang digunakan sebagai kontrol positif adalah asam askorbat dengan konsentrasi 1 ppm, 2,5 ppm, 5 ppm, 7,5 ppm dan 10 ppm, sedangkan larutan yang digunakan sebagai kontrol negatif adalah DPPH di dalam etanol absolut. Hasil penelitian menunjukkan bahwa nilai IC50 untuk ekstrak ranting lagundi adalah 8,44 ppm sedangkan asam askorbat 2,16 ppm. Berdasarkan hasil IC50 tersebut, ranting lagundi memiliki aktivitas antioksidan lebih rendah dari asam askorbat. Kata kunci: antioksidan; ekstrak ranting lagundi; lagundi; DPPH; asam askorbat. / The antioxidant activity test of Vitex negundo L twigs extract using the 1,1-difenil- 2-pikrilhidrazil method was carried out with the aim of knowing the antioxidant power of lagundi twigs extract. The lagundi twigs used in this study were fresh twigs. The variation concentrations of lagundi twigs extract were 10 ppm, 50 ppm, 100 ppm, 150 ppm and 200 ppm. The positif control was used is ascorbic acid with variation concentrations were 1 ppm, 2.5 ppm, 5 ppm, 7.5 ppm and 10 ppm, while DPPH solution in ethanol absolute was the negative control. The relusts showed that IC50 of fresh lagundi twigs were 8,44 ppm, while ascorbic acid was 2.16 ppm. Based on these IC50 values, lagundi twigs antioxidant activity was lower than ascorbic acid. Keywords : antioxidant; extract lagundi twigs; lagundi; DPPH ; ascorbic acid.

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