Uji Efektivitas Ekstrak Lidah Buaya (Aloe Vera) terhadap Pertumbuhan Escherichia Coli Secara in Vitro

Putri, Aurum Rizky (2019) Uji Efektivitas Ekstrak Lidah Buaya (Aloe Vera) terhadap Pertumbuhan Escherichia Coli Secara in Vitro. S1 thesis, Universitas Kristen Indonesia.

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Abstract

Lidah Buaya (Aloe vera) merupakan salah satu tanaman yang digunakan sebagai obat yang mempunyai berbagai khasiat, salah satunya sebagai antibakteri. Senyawa aktif yang terkandung dalam Aloe vera diantaranya seperti saponin, tannin dan flavonoid. Penelitian sebelumnya menunjukkan bahwa ekstrak lidah buaya dapat menghambat pertumbuhan bakteri gram positif dan gram negatif. Tujuan penelitian ini adalah mengetahui efek antibakteri ekstrak lidah buaya terhadap pertumbuhan Escherichia coli secara in vitro. Ekstrak Aloe vera dapat dihasilkan melalui metode maserasi dengan menggunakan lidah buaya segar dalam lima konsentrasi (20%, 40%, 60%, 80% dan 100%) yang diuji daya hambatnya. Penelitian ini menggunakan bakteri Escherichia coli yang dibiakkan dalam agar EMB dan diuji menggunakan metode difusi Kirby Bauer. Diperoleh hasil bahwa terdapat zona bening di sekitar cakram yang telah mengandung ekstrak lidah buaya pada konsentrasi 100%. Dengan demikian, ekstrak Aloe vera terbukti memiliki efektivitas antibakteri dalam menghambat pertumbuhan koloni Escherichia coli secara in vitro. Kata kunci: Ekstrak, Aloe vera, Escherichia coli. / Aloe vera is kind of living pharmacy and has an antibacterial power. The antibacterial subtances are saponin, tannin and flavonoid. Previous studies indicate that Aloe vera extracts has antibacterial effects against the growth of positive Gram bacteria either do the growth of negative Gram bacteria. The experiment was intend to observe the antibacterial effects of Aloe vera against Escherichia coli colonies. The Aloe vera extract can be done through maseration methods in five concentrations (20%, 40%, 60%, 80% dan 100%). The experiments was using Escherichia coli which perfomed on EMB agar by Kirby Bauer disc diffusion. Result showed there is clear zone near the discs that were contain of Aloe vera extracts in 100% concentration. So, it can be conclude that Aloe vera has an antibacterial effectivities against the growth of Escherichia coli colonies in vitro. Keywords: Extracts, Aloe vera, Escherichia col

Item Type: Thesis (S1)
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorParnomo, TrimurtiUNSPECIFIEDUNSPECIFIED
Subjects: MEDICINE
Divisions: FAKULTAS KEDOKTERAN > Pendidikan Dokter
Depositing User: Mr Andika Prima
Date Deposited: 04 Sep 2024 03:56
Last Modified: 04 Sep 2024 03:56
URI: http://repository.uki.ac.id/id/eprint/16422

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