Bioaktivitas Ekstrak Bunga Telang (Clitoria Ternatea L) Secara In Vitro

Sonda, Angelina Natasya (2024) Bioaktivitas Ekstrak Bunga Telang (Clitoria Ternatea L) Secara In Vitro. S1 thesis, Universitas Kristen Indonesia.

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Abstract

Bunga telang (Clitoria ternatea L) adalah salah satu tanaman herbal yang termasuk dalam famili Fabaceae. Penelitian yang dilaksanakan bertujuan untuk mengetahui tingkat toksisitas, kadar fenolik dan flavanoid serta kandungan antioksidan dari ekstrak etanol 70% serta ekstrak air bunga telang. Uji toksisitas dilakukan menggunakan metode BSLT (Brine Shrimp Lethality Test), penetapan kadar flavonoid menggunakan metode kolorimetri AlCl3, kadar fenolik ditetapkan menggunakan reagen Folin-Ciocalteu secara spektrofotometri UV-Vis serta untuk mengetahui aktivitas antioksidan dilakukan memakai metode 2,2-diphenyl-l-picrylhydrazyl (DPPH) serta ferric reducing antioxidant power (FRAP). Hasil uji toksisitas ekstrak etanol 70% serta ekstrak air bunga telang menunjukkan nilai LC50 masing-masing sebesar 61,612 ppm dan 76,475 ppm. Total fenolik pada ekstrak etanol 70% bunga telang diperoleh sejumlah 149.49776 mg/g dan ekstrak air sebesar 6.349mg/g. Total flavanoid pada ekstrak etanol 70% bunga telang diperoleh sejumlah 2.18% dan pada ekstrak air diperoleh sebesar 0.13%. Berdasarkan hasil penelitian pengujian aktivitas antioksidan dengan metode DPPH menunjukkan ekstrak etanol 70% bunga telang mempunyai aktivitas antioksidan yang tergolong rendah dengan nilai IC50 sebesar 599.88 ppm, dan vitamin C didapatkan hasil yang tergolong sangat kuat sebesar 6.31 ppm. Hasil pengujian aktivitas antioksidan dengan metode FRAP didapatkan ekstrak etanol 70% bunga telang mempunyai aktivitas antioksidan yang tergolong rendah dengan nilai IC50 sebesar 3625.065 ppm, sementara itu vitamin C sebagai pembanding sejumlah 16.492 ppm. Hasil tersebut menunjukkan jika bunga telang memiliki aktivitas antioksidan yang lemah./The telang flower (Clitoria ternatea L) is one of the herbal plants belonging to the Fabaceae family. The research conducted aims to determine the levels of toxicity, total phenolics and flavanoids as well as the antioxidant content of 70% ethanol extract and telang flower water extract. The toxicity test was carried out using the BSLT (Brine Shrimp Lethality Test) method, determination of total flavonoids using the AlCl3 colorimetry method, phenolic levels were determined using the Folin-Ciocalteu reagent using UV-Vis spectrophotometry and to determine antioxidant activity was carried out using the 2,2-diphenyl-l-picrylhydrazyl (DPPH) method and ferric reducing antioxidant power (FRAP). The toxicity test results of 70% ethanol extract and telang flower water extract showed LC50 values of 61,612 ppm and 76,475 ppm, respectively. The total phenolic in 70% ethanol extract of telang flowers was obtained at 149497.76 mg/Kg and water extract at 6349.00 mg/Kg. The total flavanoid in 70% ethanol extract of telang flowers was obtained at 2.18% and in water extract was obtained at 0.13%. Based on the results of research on antioxidant activity testing with the DPPH method, it shows that 70% ethanol extract of telang flowers has a relatively low antioxidant activity with an IC50 value of 599.88 ppm, while the telang flower water extract cannot be determined in the concentration range tested. The results of the antioxidant activity test with the FRAP method of 70% ethanol extract of Telang flowers have a relatively low antioxidant activity with an IC50 value of 3625.065 ppm, while the result of IC50 in vitamin C as a comparison of 16.492 ppm. The results show that telang flowers have weak antioxidant activity.

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