Forkhead Box P3 Messenger-RNA Expression after Curcuma longa Extract Intervention in Early Pregnant Mice with Toxoplasmosis

Simanjuntak, Tigor Peniel and Hatta, Mochammad and Rauf, Syahrul and Yusuf, Irawan and Tahir, Mardiah (2018) Forkhead Box P3 Messenger-RNA Expression after Curcuma longa Extract Intervention in Early Pregnant Mice with Toxoplasmosis. Research Journal of Immunology, 11 (1). pp. 1-6. ISSN 1994-7909

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Abstract

Background and Objective: Curcuma longa (C. Longa ) has strong anti-inflammatory effect. This study aims to examine the effect of Curcuma longa extract on Forkhead Box P3 (FOXP3) mRNA expression in early pregnant mice with acute toxoplasmosis. Materials and Methods: This study evaluated 20 early pregnant mice that were divided into 5 groups, four mice in each. Group 1-4 received injections of Toxoplasma gondii tachyzoites. Three days later, G1 and G2 were given orally 125 and 500 mg kgG1/day of Curcuma longa extract, respectively. The G3 was given 60 mg kgG1/day of spiramycin (positive control) and G4 was given 0.2 mL of distilled water (negative control). The G5 underwent no intervention at all. Blood samples were obtained serially (before and 3 days after injection of tachyzoites, 3 and 7 days after intervention) to assess FOXP3 mRNA expression. Results: The FOXP3 mRNA expression increased significantly in G1-G3 3 days after intervention (p<0.05), whereas, FOXP3 mRNA expression decreased significantly (p<0.05) 7 days after intervention and there was no significant difference between these three groups. The FOXP3 mRNA expression in G4 increased significantly 3, 6 and 10 days after tachyzoites injections, while FOXP3 mRNA expression on G5 fluctuated but considered as insignificant (p>0.05). Conclusion: The administration of Curcuma longa extract at a dose of 125 mg kgG1/day for 3 days effectively increased FOXP3 mRNA expression and 7 days administration resulted in decreased FOXP3 mRNA expression in early pregnant mice with acute toxoplasmosis. Key words: FOXP3 mRNA, Curcuma longa , pregnancy, Toxoplasma gondii , spiramycin

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