POTENSI SEL PUNCA MESENKIM ASAL JARINGAN ADIPOSA MENJADI SEL OSTEOBLAS

Bratakencana, Jennifer and Abbas, Rilianawati and Harlim, Ago (2017) POTENSI SEL PUNCA MESENKIM ASAL JARINGAN ADIPOSA MENJADI SEL OSTEOBLAS. In: Prosiding : Innovation of Bioeconomics on Agroindustrial and Biotechnology Programme. BPPT Press, Serpong, pp. 51-55. ISBN 975 602 410 097 1

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Abstract

Human adipose tissue is a great source of mesenchymal stem cells. Adipose-derived mesenchymal stem cells (AD-MSCs) are easily isolated, can differentiate into multi-lineage cells and have various clinical application. Commonly, the osteogenic differentiation of MSC were induced using culture medium that was supplemented with L-ascorbic acid 2- phosphate (Asc), dexamethasone (Dex) and beta-glycerophosphate (β-GP). The supplement composition of osteogenic medium that was frequently used (OM1 and OM2) was originally generated for bone marrow-derived mesenchymal stem cells, thus the supplement concentration may not be optimal for the differentiation of AD-MSCs. Therefore, in this study we investigated the ability of human AD-MSCs to differentiate into osteogenic lineage using four different osteogenic medium (OM1, OM2, OM3 and OM4). Cells were cultured in osteogenic medium and characterized on day 5. The result suggest that differentiation was enhanced on OM3 and OM4, which have high Asc and low Dex concentrations. Hence higher concentration of Asc, lower concentration of Dex and higher serum concentrations should be used for the osteogenic differentiation of human AD-MSCs in vitro.

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