Beban Jamur Candida dalam Saluran Cerna Anak

Hutabarat, Hardi (2016) Beban Jamur Candida dalam Saluran Cerna Anak. S1 thesis, Universitas Kristen Indonesia.

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Abstract

Mikrobiota pada saluran cerna terdiri dari bakteri, virus, jamur dan lainnya. Candida menjadi salah satu bagian dari mikrobiota pada saluran cerna anak dan bersifat saprofit tetapi dapat menjadi patogen ketika terjadi penurunan imunitas pejamu dan bila mengalami translokasi dari saluran cerna dapat mengakibatkan infeksi lokal maupun sistemik. Penelitian ini bertujuan untuk mengetahui keragaman jamur dan beban jamur pada saluran cerna anak. Dilakukan pembiakan menggunakan Agar Sabouraud Desktrosa dan CHROMagar terhadap 61 sampel tinja yang berasal dari anak sehat dan sakit. Pertumbuhan Candida sebesar 70,5% (43 sampel) dan tidak ada perbedaan yang bermakna antara beban jamur yang tumbuh pada ASD dan CHROMagar. Mayoritas (29,5%) beban jamur sebesar 1-50 CFU/g tinja dan 8,2% dari total sampel didapatkan beban jamur dengan jumlah koloni tidak terhitung. Spesies yang paling banyak diidentifikasi adalah C. tropicalis (41,6%) dan sebanyak 32,7% sampel didapatkan pertumbuhan spesies campuran. Kata Kunci: Mikrobiota, Candida, Jamur, Beban Jamur. / Microbiota in digestive tract consist of bacteria, virus, fungi and etc. Candida is a saprophyte in children digestive tract. However, it is a pathogen in person with declined immune system and also can caused local or systemic infection if translocated from digestive tracts. This study aim to determine the diversity of fungi and fungal burden in children digestive tract. Breeding of fungi performed involving 61 stool from healthy and sick children using Sabouraud Dextrose Agar and CHROMagar. Candida grow in 43 samples (70,5%) and there was no significant difference between fungal burden that growth on SDA and CHROMagar. The majority (29,5%) of fungal burden in the amount of 1-50 CFU/g stool and 8,2% of total sample were obtained fungal burden with the amount of colonies that are not counted. The most common species identified was C. tropicalis (41,6%) and 32,7% of the obtained sample was mixture of species. Keyword: Microbiome, Candida, Fungi, Fungal Burden.

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