Animal Modeling Try Strain Balb/c Mice with Gardnerella Vaginalis

Sirait, Lenny Irmawaty and Kamelia, Emma and Simanjuntak, Tigor Peniel and Massi, Muhammad Nasrum and Rauf, Syahrul and Natzir, Rosdiana and Hatta, Mochammad and Rahayu, Sri and Aritonang, Tetty Rina (2017) Animal Modeling Try Strain Balb/c Mice with Gardnerella Vaginalis. International Journal of Sciences: Basic and Applied Research (IJSBAR), 34 (1). pp. 261-267. ISSN 2307 4531

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Abstract

Gardnerella vaginalis is the causative agent of sexually transmitted infections (STIs) of the bacterial class and is the main species of bacterial vaginosis (BV), classified as gram-negative bacteria, and is anaerobic facultative. The incidence of bacterial vaginosis increased rapidly in Indonesia with a prevalence of approximately 20-30% of women of childbearing age suffering from sexually transmitted infections diagnosed BV and an increase of 50-60%. BV is the leading cause of vaginal infection in women of childbearing age and is one of the most common infectious diseases in the gynecological obstetrics. Balb-C is a trying animal with a human-like cervical vagina. The Objective of this study is to identify the dose of Gardnerella vaginalis bacteria that has the potential to live on the vagina of experimental animals. Balb/c female strain, adult age 8-12 weeks, weight 20-25 g. Using the patient's urine specimen suspect Bacterial vaginosis was inoculated on intravaginal Balb-c each of 10 μl doses of 3 x 106, 3 x 105, and 3 x 104. Prior to inoculation (H0), a vaginal swab was taken and continued at H1 ± 18-24 h post-infection. After inoculation, 3x checks per day to control the health status include movement, respiration, food, fur state (neat or messy), for a period of 5 days and weight measured 1 day before and 5 days after infection. Vaginal ecosystem changes 18-24 hours post inoculation of intravaginal Gardnerella vaginalis indicated by growth of cultured culture colony on Plat count agar (PCA) ie 21 cfu / ml for dose 3 x 104; 106 cfu / ml for a dose of 3 x 105; And too much to count (± 10000) for a dose of 3 x 106. In conclusions; the dose `Concentration 3 x 104 can be recommended as a concentration of treatment of infection of experimental animals using Gardnerella vaginalis in subsequent studies

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