Bioaktivitas Daun Kemangi (Ocimum Sanctum) dan Daun Ruku Ruku (Ocimum Tenuiflorum)

Kurniawan, Kelvin (2020) Bioaktivitas Daun Kemangi (Ocimum Sanctum) dan Daun Ruku Ruku (Ocimum Tenuiflorum). S1 thesis, Universitas Kristen Indonesia.

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Abstract

Kemangi (Ocimum sanctum) merupakan salah satu tanaman yang biasa dimanfaatkan sebagai sayur (lalapan) dan obat tradisional di Indonesia. Secara empiris daun kemangi telah digunakan sebagai obat alternatif untuk penyembuhan luka. Selain kemangi, tanaman yang secara morfologi memiliki kesamaan dengan kemangi yaitu ruku ruku juga sering digunakan oleh masyarakat Indonesia sebagai bumbu masakan. Daun ruku ruku (Ocimum tenuiflorum) secara empiris digunakan dalam berbagai pengobatan alternatif seperti mengobati demam, batuk, encok, urat syaraf, sariawan, panu, mual dan muntah-muntah. Penelitian yang dilakukan bertujuan untuk mengetahui aktivitas antimikroba (antibakteri dan antijamur), antioksidan serta analisis fitokimia dari ekstrak etanol daun kemangi dan daun rukuruku. Metode ekstraksi yang dipakai adalah metode maserasi dengan mengunakan pelarut etanol 96%. Uji antimikroba yang dilakukan menggunakan metode difusi agar. Bakteri yang digunakan adalah Salmonella typhi, Staphylococcus aureus sedangkan jamur yang digunakan adalah Candida albicans. Analisis fitokimia menggunakan metode Harbone, dan uji antioksidan menggunakan metode 1,1- Diphenyl-2-picryl Hidrazil (DPPH). Hasil penelitian menunjukkan bahwa ekstrak daun kemangi dan daun ruku ruku mengandung senyawa golongan flavonoid dan steroid. Hasil uji anti mikroba menunjukkan bahwa hanya daun ruku ruku yang menghambat pertumbuhan Staphylococcus aureus dengan zona hambat 9,35 mm dan kedua ekstrak yang digunakan tidak dapat menghambat pertumbuhan jamur Candida albicans. Sedangkan hasil uji antioksidan menunjukkan bahwa ekstrak daun ruku ruku dan daun kemangi memiliki aktivitas antioksidan dengan nilai inhibition concentration 50 (IC50) masing-masing sebesar 225,64 ppm dan 455,11 ppm. Kata kunci: Bioaktivitas, Fitokimia, Kemangi, Ruku Ruku. / Kemangi (Ocimum sanctum) is a plant commonly used as a vegetable (fresh vegetables) and traditional medicine in Indonesia. Kemangi leaves have been empirically used as an alternative medicine for wound healing. Apart from kemangi, a plant that is morphologically similar to kemangi, namely ruku ruku, is also often used by Indonesians as a cooking spice. The leaves of ruku ruku (Ocimum tenuiflorum) have been empirically used in various alternative medicine, namely to treat fever, cough, gout, nerves, mouth sores, tinea versicolor, nausea and vomiting. The research was conducted to determine the antimicrobial activity (antibacterial and antifungal), antioxidants and phytochemical analysis of the ethanol extract of kemangi leaves and ruku ruku leaves. The extraction method used is the maceration method using 96% ethanol as a solvent. The antimicrobial test was carried out using the agar diffusion method. The bacteria used are Salmonella typhi, Staphylococcus aureus while the fungi used are Candida albicans. Phytochemical analysis used the Harbone method, and the antioxidant test used the 1,1-Diphenyl-2-picryl Hidrazil (DPPH) method. The results showed that the extracts of kemangi and ruku ruku leaves contained flavonoid and steroid compounds. The results of the anti-microbial test showed that only ruku ruku leaves inhibited the growth of Staphylococcus aureus with an inhibition zone of 9.35 mm and the two extracts used could not inhibit the growth of the fungus Candida albicans. While the results of the antioxidant test showed that the ruku ruku and kemangi leaf extracts had antioxidant activity with inhibition concentration 50 (IC50) values of 225.64 ppm and 455.11 ppm. Keywords: Bioactivity, Kemangi, Phytochemicals, Ruku ruku.

Item Type: Thesis (S1)
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorAlfarabi, MuhammadUNSPECIFIEDUNSPECIFIED
Subjects: MEDICINE
Divisions: FAKULTAS KEDOKTERAN > Pendidikan Dokter
Depositing User: Mr Andika Prima
Date Deposited: 04 Sep 2024 02:44
Last Modified: 04 Sep 2024 02:44
URI: http://repository.uki.ac.id/id/eprint/16589

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