Biochemical Aspects of Cell Staining

Suryowati, Trini (2024) Biochemical Aspects of Cell Staining. Asian Journal of Research in Biochemistry, 14 (3). pp. 61-71. ISSN 2582-0516

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Abstract

Aims: to revisited the biochemical aspects of basic, negative and positive staining by dyeing organic materials in the laboratory for the purpose of visualization, identification and even differentiation of object being analyzed. Discussion: The best analysis for cells is by staining. Staining allows detailed visualization of the part of the cell being examined. In principle, dyes have a chromophore root that will bind to the protein content in cells. This is the basis for discussing negative staining, basic staining and positive staining. Unfortunately, its widespread use also increases the health risks of users. Conclusion: Biochemical aspect of staining rely on the electric charged of the dye that bind the electronegative properties of proteins that build cell. Basic staining, negative staining and positive staining are widely used staining for research, clinical-laboratory service and also education. Unfortunately, some of these stains are hazardous with the potency of multiple health risk and continuous improvement in handling will prevent such risk to happen. Keywords: Cellular imaging; dye; organic material; positive; negative; laboratory.

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