Interaction between Dimethylpolysiloxane and Autologous Plasma Triggering in Vitro Inflammation in Cultured PBMC

Harlim, Ago and F, Mujadid and H, Wibowo (2018) Interaction between Dimethylpolysiloxane and Autologous Plasma Triggering in Vitro Inflammation in Cultured PBMC. GRF Publisher, 2018 (1). pp. 1-6.

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Abstract

The data obtained from this study try to explain the mechanism of cellular immune response of dmps-injected recipients with in vitro-based method to get the description of immune responses that occurs in the body due to exposure to dmps which can lead to granuloma formation, followed by fibrosis. pbmc is taken from normal patients and patients with granulomas due to injection of dmps. And then, it was cultured for72 hours with rpmi treatment as a negative control, pha and lpsas a positive control, dmps only and dmps with the addition of autologous plasma. The purpose of the pbmc culture was to describe the activity of tnf-a, ifn-γ, il-6, il13, il-10 and pbmc proliferation. There was no significant increase (p> 0.05) in proliferation of lymphocytes and monocytes in the dmps-treated group, both in normal patients and patients with granulomas. The role of autologous plasma was not observed in increasing cell proliferation. Nonetheless, autologous plasma had a role in increasing activity of tnf-a and il-6 significantly (p <0.05) in response to dmps exposure in both of patients. The data of this study indicated that dmps was able to trigger non specific inflammatory activity mediated by tnf-a and il-6 and it depended on each individual plasma proteins. Specific cellular immune response due to dmps exposure, as lymphocyte and monocyte proliferation, was not observed

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